Review



mouse anti-sheep cd4 44.38  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Bio-Rad mouse anti-sheep cd4 44.38
    Lymphocytes traveling in afferent lymph draining uninflamed or chronically inflamed skin (>d15 post induction of inflammation with CFA) were collected from sheep, and chemotaxis was tested toward mouse CCL21 and human CXCL12 in a Transwell chemotaxis assay. Migration of <t>CD4,</t> CD8 and γδ T cells is expressed as the percentage of each T cell subset that migrated to the lower chamber. Data points represent the mean migration of T cells from individual animals based on triplicate wells for each concentration, and the mean ± SEM for all animals analyzed is shown. N = 5–7 animals per condition and T cell subset.
    Mouse Anti Sheep Cd4 44.38, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti-sheep cd4 44.38/product/Bio-Rad
    Average 90 stars, based on 1 article reviews
    mouse anti-sheep cd4 44.38 - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "CXCR4 Is Dispensable for T Cell Egress from Chronically Inflamed Skin via the Afferent Lymph"

    Article Title: CXCR4 Is Dispensable for T Cell Egress from Chronically Inflamed Skin via the Afferent Lymph

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0095626

    Lymphocytes traveling in afferent lymph draining uninflamed or chronically inflamed skin (>d15 post induction of inflammation with CFA) were collected from sheep, and chemotaxis was tested toward mouse CCL21 and human CXCL12 in a Transwell chemotaxis assay. Migration of CD4, CD8 and γδ T cells is expressed as the percentage of each T cell subset that migrated to the lower chamber. Data points represent the mean migration of T cells from individual animals based on triplicate wells for each concentration, and the mean ± SEM for all animals analyzed is shown. N = 5–7 animals per condition and T cell subset.
    Figure Legend Snippet: Lymphocytes traveling in afferent lymph draining uninflamed or chronically inflamed skin (>d15 post induction of inflammation with CFA) were collected from sheep, and chemotaxis was tested toward mouse CCL21 and human CXCL12 in a Transwell chemotaxis assay. Migration of CD4, CD8 and γδ T cells is expressed as the percentage of each T cell subset that migrated to the lower chamber. Data points represent the mean migration of T cells from individual animals based on triplicate wells for each concentration, and the mean ± SEM for all animals analyzed is shown. N = 5–7 animals per condition and T cell subset.

    Techniques Used: Chemotaxis Assay, Migration, Concentration Assay

    ( A–C ) Adoptive transfer exit experiments with fetal liver chimeric mice as a source of donor splenocytes. As depicted in the experimental scheme ( A ), fetal liver chimeras were generated by reconstituting lethally irradiated CD45.1 + wildtype recipient mice with fetal liver cells from (CD45.2 + ) Cxcr4 −/− Ccr 7 −/− or (CD45.2 + ) Ccr7 −/− or (CD45.1 + ) wildtype donors. Splenocytes from the fetal liver chimeras were labeled with eFlour670 and CFSE, so that the combination of congenic maker and fluorescent label uniquely marked cells of each genotype. Labeled splenocytes of all three genotypes were mixed and co-injected into chronically inflamed footpads. ( B ) Analysis of migrated lymphocyte subsets recovered in the draining popliteal lymph nodes 12 h post cell transfer. Data is presented as the ratio of migrated to input cells for Ccr7 −/− or Cxcr4 −/− Ccr 7 −/− relative to wildtype cells of each subset. Connected lines represent individually analyzed recipient mice. Horizontal, non-connecting lines indicate the mean of each group. ( C ) Flow cytometric analysis of memory (CD45RB l °) versus naïve (CD45RB hi ) phenotype CD4 and CD8 T cells in injected and migrated populations for each genotype. Data combined from 2 experiments analyzing 5–10 recipient mice per experiment ( B ) or the cell phenotype of one out two experiment with similar results ( C ) is shown. WT, wildtype.
    Figure Legend Snippet: ( A–C ) Adoptive transfer exit experiments with fetal liver chimeric mice as a source of donor splenocytes. As depicted in the experimental scheme ( A ), fetal liver chimeras were generated by reconstituting lethally irradiated CD45.1 + wildtype recipient mice with fetal liver cells from (CD45.2 + ) Cxcr4 −/− Ccr 7 −/− or (CD45.2 + ) Ccr7 −/− or (CD45.1 + ) wildtype donors. Splenocytes from the fetal liver chimeras were labeled with eFlour670 and CFSE, so that the combination of congenic maker and fluorescent label uniquely marked cells of each genotype. Labeled splenocytes of all three genotypes were mixed and co-injected into chronically inflamed footpads. ( B ) Analysis of migrated lymphocyte subsets recovered in the draining popliteal lymph nodes 12 h post cell transfer. Data is presented as the ratio of migrated to input cells for Ccr7 −/− or Cxcr4 −/− Ccr 7 −/− relative to wildtype cells of each subset. Connected lines represent individually analyzed recipient mice. Horizontal, non-connecting lines indicate the mean of each group. ( C ) Flow cytometric analysis of memory (CD45RB l °) versus naïve (CD45RB hi ) phenotype CD4 and CD8 T cells in injected and migrated populations for each genotype. Data combined from 2 experiments analyzing 5–10 recipient mice per experiment ( B ) or the cell phenotype of one out two experiment with similar results ( C ) is shown. WT, wildtype.

    Techniques Used: Adoptive Transfer Assay, Generated, Irradiation, Labeling, Injection



    Similar Products

    mouse anti-sheep cd4 44.38  (Bio-Rad)
    90
    Bio-Rad mouse anti-sheep cd4 44.38
    Lymphocytes traveling in afferent lymph draining uninflamed or chronically inflamed skin (>d15 post induction of inflammation with CFA) were collected from sheep, and chemotaxis was tested toward mouse CCL21 and human CXCL12 in a Transwell chemotaxis assay. Migration of <t>CD4,</t> CD8 and γδ T cells is expressed as the percentage of each T cell subset that migrated to the lower chamber. Data points represent the mean migration of T cells from individual animals based on triplicate wells for each concentration, and the mean ± SEM for all animals analyzed is shown. N = 5–7 animals per condition and T cell subset.
    Mouse Anti Sheep Cd4 44.38, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti-sheep cd4 44.38/product/Bio-Rad
    Average 90 stars, based on 1 article reviews
    mouse anti-sheep cd4 44.38 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Lymphocytes traveling in afferent lymph draining uninflamed or chronically inflamed skin (>d15 post induction of inflammation with CFA) were collected from sheep, and chemotaxis was tested toward mouse CCL21 and human CXCL12 in a Transwell chemotaxis assay. Migration of CD4, CD8 and γδ T cells is expressed as the percentage of each T cell subset that migrated to the lower chamber. Data points represent the mean migration of T cells from individual animals based on triplicate wells for each concentration, and the mean ± SEM for all animals analyzed is shown. N = 5–7 animals per condition and T cell subset.

    Journal: PLoS ONE

    Article Title: CXCR4 Is Dispensable for T Cell Egress from Chronically Inflamed Skin via the Afferent Lymph

    doi: 10.1371/journal.pone.0095626

    Figure Lengend Snippet: Lymphocytes traveling in afferent lymph draining uninflamed or chronically inflamed skin (>d15 post induction of inflammation with CFA) were collected from sheep, and chemotaxis was tested toward mouse CCL21 and human CXCL12 in a Transwell chemotaxis assay. Migration of CD4, CD8 and γδ T cells is expressed as the percentage of each T cell subset that migrated to the lower chamber. Data points represent the mean migration of T cells from individual animals based on triplicate wells for each concentration, and the mean ± SEM for all animals analyzed is shown. N = 5–7 animals per condition and T cell subset.

    Article Snippet: The following mouse anti-sheep mAbs were used: CD4 (clone 44.38; Serotec), CD8 (clone 38.65; Serotec), and γδ TCR (clone 86D; VMRD).

    Techniques: Chemotaxis Assay, Migration, Concentration Assay

    ( A–C ) Adoptive transfer exit experiments with fetal liver chimeric mice as a source of donor splenocytes. As depicted in the experimental scheme ( A ), fetal liver chimeras were generated by reconstituting lethally irradiated CD45.1 + wildtype recipient mice with fetal liver cells from (CD45.2 + ) Cxcr4 −/− Ccr 7 −/− or (CD45.2 + ) Ccr7 −/− or (CD45.1 + ) wildtype donors. Splenocytes from the fetal liver chimeras were labeled with eFlour670 and CFSE, so that the combination of congenic maker and fluorescent label uniquely marked cells of each genotype. Labeled splenocytes of all three genotypes were mixed and co-injected into chronically inflamed footpads. ( B ) Analysis of migrated lymphocyte subsets recovered in the draining popliteal lymph nodes 12 h post cell transfer. Data is presented as the ratio of migrated to input cells for Ccr7 −/− or Cxcr4 −/− Ccr 7 −/− relative to wildtype cells of each subset. Connected lines represent individually analyzed recipient mice. Horizontal, non-connecting lines indicate the mean of each group. ( C ) Flow cytometric analysis of memory (CD45RB l °) versus naïve (CD45RB hi ) phenotype CD4 and CD8 T cells in injected and migrated populations for each genotype. Data combined from 2 experiments analyzing 5–10 recipient mice per experiment ( B ) or the cell phenotype of one out two experiment with similar results ( C ) is shown. WT, wildtype.

    Journal: PLoS ONE

    Article Title: CXCR4 Is Dispensable for T Cell Egress from Chronically Inflamed Skin via the Afferent Lymph

    doi: 10.1371/journal.pone.0095626

    Figure Lengend Snippet: ( A–C ) Adoptive transfer exit experiments with fetal liver chimeric mice as a source of donor splenocytes. As depicted in the experimental scheme ( A ), fetal liver chimeras were generated by reconstituting lethally irradiated CD45.1 + wildtype recipient mice with fetal liver cells from (CD45.2 + ) Cxcr4 −/− Ccr 7 −/− or (CD45.2 + ) Ccr7 −/− or (CD45.1 + ) wildtype donors. Splenocytes from the fetal liver chimeras were labeled with eFlour670 and CFSE, so that the combination of congenic maker and fluorescent label uniquely marked cells of each genotype. Labeled splenocytes of all three genotypes were mixed and co-injected into chronically inflamed footpads. ( B ) Analysis of migrated lymphocyte subsets recovered in the draining popliteal lymph nodes 12 h post cell transfer. Data is presented as the ratio of migrated to input cells for Ccr7 −/− or Cxcr4 −/− Ccr 7 −/− relative to wildtype cells of each subset. Connected lines represent individually analyzed recipient mice. Horizontal, non-connecting lines indicate the mean of each group. ( C ) Flow cytometric analysis of memory (CD45RB l °) versus naïve (CD45RB hi ) phenotype CD4 and CD8 T cells in injected and migrated populations for each genotype. Data combined from 2 experiments analyzing 5–10 recipient mice per experiment ( B ) or the cell phenotype of one out two experiment with similar results ( C ) is shown. WT, wildtype.

    Article Snippet: The following mouse anti-sheep mAbs were used: CD4 (clone 44.38; Serotec), CD8 (clone 38.65; Serotec), and γδ TCR (clone 86D; VMRD).

    Techniques: Adoptive Transfer Assay, Generated, Irradiation, Labeling, Injection